Manufacturing and Characterization of Poly(Hydroxybutyrate)/Multiwalled Carbon Nanotubes Nanocomposite Membrane for the Treatment of Bone Defects and Oral Tissue Regeneration
Paper ID : 1229-MST2015-FULL
1moein zarei *, 2saeed karbasi, 3mohammad foroughi
1Faculty of Science and Nuclear Engineering, Islamic Azad University, Najafabad branch, Isfahan, Iran
2Medical Physics and Biomedical Engineering Group, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3Dental Materials Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
A series of poly(hydroxybutyrate) (PHB)/Multiwalled Carbon Nanotubes (MWNTs) nanocomposite scaffolds was prepared by electrospinning for tissue engineering applications. The effect of MWNTs content on the structure and properties of composite membrane was characterized by scanning electron microscopy (SEM) and universal testing machine (UTM). The attachment ability and viability of osteosarcoma cell lines (MG-63) in the presence of the scaffolds were also investigated. Morphological characterization showed that the addition of different amounts of MWNTs increased the average fiber diameter. The analysis of mechanical properties of the PHB/MWNTs composites revealed great improvement over pure PHB scaffold. The attachment and proliferation of MG-63 Cells were significantly increased in the PHB/MWNTs scaffolds compared with the PHB control. Therefore, the PHB/MWNTs composite scaffolds fabricated by electrospinning may be potentially useful in tissue engineering applications, particularly as membrane for bone and oral tissue regeneration.
PHB, MWNTs, Electrospinning, Membrane , Scaffold, Tissue Regeneration
Status : Paper Accepted (Oral Presentation)